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Image Search Results
Journal: Immunity
Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development
doi: 10.1016/j.immuni.2020.06.006
Figure Lengend Snippet: Immunoglobulin heavy chain gene-sequencing of single GAC+ B cells depicted as Circos plots (See Experimental Procedures). (A) GAC+ B cells (n=94) sorted from naïve adult C57BL/6 mice, (B) Adult C57BL/6 mice were immunized with GAS at d56 and GAC+ B cells were sorted 14 days post-immunization (d.p.i.) (n=74), (C) GAC+ B cells sorted from d56 resting adult C57BL/6 mice previously immunized as pups at d14 (n=87). IGH sequences are pooled from naïve (n=4), d56-immunized (n=4) or d14-immunized (n=4) mice from one representative experiment completed twice. Ribbon color coresponds IGHV identity (IGHV6 blue, IGHV7–3 red, all other IGHVs grey), IGHJ usage and IGHV mutations are depicted by inlayed heatmaps. (D) CDRH3 length distribution of pooled IGH gene sequences of identifiied IGHV families of GAC+ B cells. (E) CDRH3 consensus logos of of the mode CDRH3 length for pooled IGHV6–3 and IGHV7–3 IGH gene sequences. See also Figure S2. (F) Relative IGHV gene abundance in recall responses elicited from adult and d14 GAS-immunized mice following a prime-boost regimen. See also Figure S3. (G) Relative abundance of IGHV gene utilization of GAC+ B cells in d14, d21 and d28 neonates, adult (8–10wk) mice and aged adult (10–12mo) mice. (H) Relative IGHV gene abundance in AA4.1+ immature and AA4.1− mature GAC+ B cells isolated from d28 neonates. See also Figure S3.
Article Snippet:
Techniques: Sequencing, Isolation
Journal: Immunity
Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development
doi: 10.1016/j.immuni.2020.06.006
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, Sequencing, Software